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KMID : 1007520140230051695
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Food Science and Biotechnology
2014 Volume.23 No. 5 p.1695 ~ p.1701
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Ethylacetate Extracts of the Muscles of Anguilla japonica Suppress Glucose Levels in db/db Mice via Activation of AMP-activated Protein Kinase
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Jeong Yong-Tae
Yang Ju-Hye
Li Xian
Kim Geum-Jin
Kim Dong-Soo
Kim Cheorl-Ho
Na Min-Kyun
Chang Hyeun-Wook
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Abstract
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Hypoglycemic effects of ethylacetate extracts of Anguilla japonica (EMA) muscles in db/db mice were investigated. To understand the mechanism responsible for the hypoglycemic effects of EMA, the effects of EMA on AMP-activated protein kinase (AMPK) activation in L6 myotubes and in vivo using type II diabetic db/db mice were analyzed. In L6 myotubes, the phosphorylation degrees of AMPK and acetyl-CoA carboxylase (ACC) were markedly increased and glucose uptake was significantly (p<0.001) increased by EMA, compared with untretaed L6 myotubes. However, in L6 myotubes, these effects were abolished by compound C, an AMPK inhibitor. Moreover, EMA significantly reduced non-fasting blood glucose and serum insulin levels, and strongly induced AMPK phosphorylation in skeletal muscle tissues of db/db mice. EMA regulates glucose levels in L6 myotubes and in diabetic mice by activation of AMPK. Beneficial effects for diabetes treatment are indicated.
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KEYWORD
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Anguilla japonica, AMP-activated protein kinase, db/db mouse, glucose uptake, L6 myotube
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